Volume 80, Issue 3 p. 277-282

Pulsed-field gel electrophoresis as an epidemiological tool for clonal identification of Aeromonas hydrophila

D. Talon

Corresponding Author

D. Talon

Institut des Sciences et des Techniques de l'environnement, Université de Franche-Comté, Rennes, France

Dr Daniel Talon, Laboratoire de Bactériologie-Hygiène, Hôpital Jean Minjoz, Bl Fleming, 25030 Besançon, France.Search for more papers by this author
M.J. Dupont

M.J. Dupont

Institut des Sciences et des Techniques de l'environnement, Université de Franche-Comté, Rennes, France

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J. Lesne

J. Lesne

Laboratorie d'Etude et de Recherche en Environment et Santé, Ecole Nationale de Santé Publique, Rennes, France

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M. Thouverez

M. Thouverez

Institut des Sciences et des Techniques de l'environnement, Université de Franche-Comté, Rennes, France

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Y. Michel-Briand

Y. Michel-Briand

Institut des Sciences et des Techniques de l'environnement, Université de Franche-Comté, Rennes, France

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First published: March 1996
Citations: 43

Abstract

Pulsedfield gel electrophoresis (PFGE) was used to characterize Aeromonas hydrophila strains isolated from a cluster of hospital-acquired infections that occurred over approximately 1 month in a French hospital. Five isolates from patients and 10 isolates from the water supply were characterized by biotyping and antibiotic susceptibility patterns and compared with 10 epidemiologically unrelated strains isolated from patients and rivers, by PFGE of digests of chromosomal DNA. Five environmental and four clinical isolates belonged to the same biotype and antibiotic susceptibility pattern type. The endonucleases XbaI, SpeI and SwaI gave satisfactory profiles whereas DraI did not. The profiles were stable, reproducible and discriminatory. The 10 epidemiologically unrelated strains exhibited 10 different patterns after digestion with XbaI, the least expensive, suitable endonuclease. PFGE is a rapid and discriminatory technique for the typing of Aeromonas hydrophila where a common origin of infection is suspected.