Selective carbohydrate utilization by lactobacilli and bifidobacteria
D. Watson
Alimentary Pharmabiotic Centre, Department of Microbiology, University College Cork, Cork, Ireland
Search for more papers by this authorM. O'Connell Motherway
Alimentary Pharmabiotic Centre, Department of Microbiology, University College Cork, Cork, Ireland
Search for more papers by this authorM.H.C. Schoterman
FrieslandCampina, Amersfoort, the Netherlands
Search for more papers by this authorR.J. Joost van Neerven
FrieslandCampina, Amersfoort, the Netherlands
Search for more papers by this authorCorresponding Author
D. van Sinderen
Alimentary Pharmabiotic Centre, Department of Microbiology, University College Cork, Cork, Ireland
Correspondence
Douwe van Sinderen, Room 4.05, Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Western Road, Cork, Ireland. E-mail: [email protected]
Search for more papers by this authorD. Watson
Alimentary Pharmabiotic Centre, Department of Microbiology, University College Cork, Cork, Ireland
Search for more papers by this authorM. O'Connell Motherway
Alimentary Pharmabiotic Centre, Department of Microbiology, University College Cork, Cork, Ireland
Search for more papers by this authorM.H.C. Schoterman
FrieslandCampina, Amersfoort, the Netherlands
Search for more papers by this authorR.J. Joost van Neerven
FrieslandCampina, Amersfoort, the Netherlands
Search for more papers by this authorCorresponding Author
D. van Sinderen
Alimentary Pharmabiotic Centre, Department of Microbiology, University College Cork, Cork, Ireland
Correspondence
Douwe van Sinderen, Room 4.05, Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Western Road, Cork, Ireland. E-mail: [email protected]
Search for more papers by this authorAbstract
Aim
To evaluate the ability of specific carbohydrates, including commercially available products, to support the growth of representatives of two well-known groups of gut commensals, namely lactobacilli and bifidobacteria.
Methods and Results
Sixty-eight bacterial strains, representing 29 human-derived lactobacilli and 39 bifidobacteria (both human- and animal-derived), were tested for their ability to metabolize 10 different carbohydrates. Analysis of growth and metabolic activity was performed using a combination of diagnostic parameters, such as final OD600, final pH, fermentation end products and growth rate.
Conclusions
The data assembled in this study provide significant complementary and comparative information on the growth-promoting properties of a range of carbohydrates, while also investigating interspecies differences between lactobacilli and/or bifidobacteria with regard to their carbohydrate utilization abilities. Galacto-oligosaccharides (GOS) and lactulose were shown to support the most favourable growth characteristics, whereas relatively poor growth of lactobacilli and bifidobacteria was observed on inulin, maltodextrin and polydextrose. GOS/inulin (9 : 1) and fructo-oligosaccharides (FOS)/inulin mixtures supported mostly similar growth abilities to those obtained for GOS and FOS, respectively. Microbial consumption of GOS, as determined by high-performance anion-exchange chromatography with pulsed amperometric detection, was evident for both lactobacilli and bifidobacteria.
Significance and Impact of the Study
These results may allow for the rational prediction of lactobacilli and/or bifidobacteria to be used in conjunction with prebiotics, such as GOS, as synbiotics.
Supporting Information
Filename | Description |
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jam12105-sup-0001-FigureS1.pdfapplication/PDF, 143.8 KB | Figure S1 (I) The HPAEC profile of the reference FOS mixture indicated an abundance of different oligosaccharides with different retention times. (II–V) Qualitative FOS degradation patterns for Bif. infantis NCDO 2205, Bif. dentium NCFB 2843, Bif. pseudolongum NCIMB 2244 and Bif. longum Onken. |
jam12105-sup-0002-TableS1.pptxapplication/, 77.2 KB | Table S1 Final OD600 values reached by lactobacilli following 24 h of growth. |
jam12105-sup-0003-TableS2.pptxapplication/, 80.4 KB | Table S2 Final OD600 values reached by bifidobacteria following 24 h of growth. |
jam12105-sup-0004-TableS3.pptxapplication/, 78.6 KB | Table S3 pH values of the growth medium following 24 h of growth by lactobacilli |
jam12105-sup-0005-TableS4.pptapplication/, 684.5 KB | Table S4 pH of growth medium following 24 h of growth of various bifidobacteria. |
jam12105-sup-0006-TableS5.pptxapplication/, 74.1 KB | Table S5 Growth rates for lactobacilli growing on various carbohydrate sources. |
jam12105-sup-0007-TableS6.pptxapplication/, 69.8 KB | Table S6 Growth rates for bifidobacteria growing on various carbohydrate sources. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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