Volume 119, Issue 6 p. 1624-1636
Original Article

Antifungal and antimycotoxigenic potency of Solanum torvum Swartz. leaf extract: isolation and identification of compound active against mycotoxigenic strains of Aspergillus flavus and Fusarium verticillioides

R.U. Abhishek

R.U. Abhishek

Department of Microbiology and Biotechnology, Bangalore University, Bengaluru, India

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S. Thippeswamy

S. Thippeswamy

Department of Microbiology and Biotechnology, Bangalore University, Bengaluru, India

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K. Manjunath

K. Manjunath

Department of Microbiology and Biotechnology, Bangalore University, Bengaluru, India

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D.C. Mohana

Corresponding Author

D.C. Mohana

Department of Microbiology and Biotechnology, Bangalore University, Bengaluru, India

Correspondence

Devihalli C. Mohana, Department of Microbiology and Biotechnology, Bengaluru University, Jnana Bharathi, Bengaluru-560 056, India.

E-mail: [email protected]

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First published: 22 September 2015
Citations: 22

Abstract

Aims

The main objective of this study was to investigate the antifungal effect of Solanum torvum leaves against different field and storage fungi, and to identify its active compound. In addition, to evaluate in vitro and in vivo inhibitory efficacy on toxigenic strains of Aspergillus flavus and Fusarium verticillioides.

Methods and Results

Leaves of S. torvum were sequentially extracted with petroleum ether, toluene, chloroform, methanol and ethanol. The antifungal compound isolated from chloroform extract was identified as torvoside K based on spectral analysis. The antifungal activity of chloroform extract and torvoside K was determined by broth microdilution and poisoned food techniques. The minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and zone of inhibition (ZOI) were recorded. Further, inhibitory effects of chloroform extract and torvoside K on growth of A. flavus and F. verticillioides, and their toxin productions were evaluated using in vitro and in vivo assays. Torvoside K showed the significant activity against tested fungi with ZOIs and MICs ranging from 33·4 to 87·4% and 31·25–250 μg ml−1, respectively. Further, torvoside K showed concentration-dependent antimycotoxigenic activity against aflatoxin B1 and fumonisin B1 production by A. flavus and F. verticillioides, respectively.

Conclusions

It was observed that the compound torvoside K significantly inhibited the growth of all fungi tested. Growth of A. flavus and F. verticillioides, and aflatoxin B1 and fumonisin B1 productions were completely inhibited in vitro and in vivo by torvoside K with increasing concentration.

Significance and Impact of the Study

Control of mycotoxigenic fungi requires compounds that able to inhibit both fungal growth and mycotoxin production. The antimycotoxigenic potential of torvoside K of S. torvum is described in this study for the first time. The results indicate the possible use of S. torvum as source of antifungal agents against postharvest fungal infestation of food commodities and mycotoxin contaminations.