Issue Information

Open Access

Issue Information

  • Pages: 483-484
  • First Published: 23 February 2023

SPECIAL ISSUE: MICROBIAL ELECTROCHEMICAL TECHNOLOGIES AND SYNTHETIC BIOLOGY

Open Access

3D printing of microbial communities: A new platform for understanding and engineering microbiomes

  • Pages: 489-493
  • First Published: 13 December 2022
Description unavailable

Many microbes naturally live in diverse, spatially structured communities, that are challenging to study and manipulate. 3D printing offers an exciting new solution to these challenges, as it can precisely arrange microbes in 3D space, allowing one to build custom microbial communities for a wide range of purposes in research, medicine, and industry.

Open Access

Marinobacter: A case study in bioelectrochemical chassis evaluation

  • Pages: 494-506
  • First Published: 04 December 2022
Description unavailable

The junction of bioelectrochemical systems and synthetic biology opens the door to many potentially groundbreaking technologies. When developing these possibilities, choosing the correct chassis organism can save a great deal of engineering effort and, indeed, can mean the difference between success and failure. In this review, we will explore the metabolic and electrochemical potential of the Marinobacter genus, suggesting applications to which these organisms may be best suited.

Open Access

Living electronics: A catalogue of engineered living electronic components

  • Pages: 507-533
  • First Published: 14 December 2022
Description unavailable

Biology leverages a range of electrical phenomena to extract and store energy, control molecular reactions and enable multicellular communication. Recently, the microbial machinery enabling these redox reactions have been leveraged for interfacing cells and biomolecules with electrical circuits for biotechnological applications. Herein, we review the state of such living electronic components including methods for assembling these components into living electronic circuits.

Open Access

Enhancing electrical outputs of the fuel cells with Geobacter sulferreducens by overexpressing nanowire proteins

  • Pages: 534-545
  • First Published: 03 August 2022
Description unavailable

Geobacter sulfurreducens strains with overexpressed nanowire proteins were constructed and used as electricigens in microbial fuel cells. Enhanced electrical outputs based on promoting biofilm formation on anode demonstrated that nanowire proteins are promising genetic tools to improve electrochemical performance of microbes for bioelectricity production.

Open Access

An electrogenetic toggle switch model

  • Pages: 546-559
  • First Published: 07 October 2022
Description unavailable

We analyse the use of electrogenic processes for circuit design and present a model for a merged genetic and electrogenetic toggle switch operating in a biofilm attached to an electrode. Computational simulations explore conditions under which bistability emerges in order to identify the circuit design principles for best switch performance. The results provide a basis for the rational design and implementation of hybrid devices that can be measured and controlled both genetically and electronically.

Open Access

NADH dehydrogenases drive inward electron transfer in Shewanella oneidensis MR-1

  • Pages: 560-568
  • First Published: 24 November 2022
Description unavailable

This study elucidates the role of NADH dehydrogenases in electron uptake from a cathode to drive intracellular acetoin reduction in Shewanella oneidensis.

Open Access

Microbial photoelectrosynthesis: Feeding purple phototrophic bacteria electricity to produce bacterial biomass

  • Pages: 569-578
  • First Published: 19 December 2022
Description unavailable

Carbon fixation through extracellular electron uptake places purple phototrophic bacteria in the field of microbial electrosynthesis as key carbon capturing microorganisms. In this work we demonstrate biomass production dominated by purple phototrophic bacteria from Rhodopseudomonas genus with a cathode (−0.6 V vs. Ag/AgCl) as electron donor.

Open Access

Screening of natural phenazine producers for electroactivity in bioelectrochemical systems

  • Pages: 579-594
  • First Published: 26 December 2022
Description unavailable

We here investigated the potential electroactivity of over one hundred putative diverse native phenazine-producers to provide a deeper understanding of the ecological potential for the bioelectrochemical exploitation of phenazines. From 104 tested strains, two showed an outstanding performance for mediated extracellular electron transfer in our BES: P. chlororaphis subsp. aurantiaca and N. chromatogenes.

Open Access

Improving the performance of bioelectrochemical sulfate removal by applying flow mode

  • Pages: 595-604
  • First Published: 19 October 2022
Description unavailable

In this study, continuous mode was applied to initial autotrophic bioelectrochemical sulfate reduction allowing a tripling of the sulfate removal rate compared to batch mode conditions which had been applied in previous studies. Microbial community analysis demonstrated the presence of a few hydrogenotrophic and heterotrophic sulfate reducers but also an increased abundance of homoacetogens, a process which is seemingly beneficial for bioelectrochemical sulfate reduction as the produced acetate could lead to an improved biomass production.

ANNOUNCEMENT

Open Access

The following article for this Special Issue were published in different Issue

  • Pages: e1
  • First Published: 23 February 2023

Ying Wang, Hongyu Chen, Liang Ma, Ming Gong, Yingying Wu, Dapeng Bao and Gen Zou. Use of CRISPR-Cas tools to engineer Trichoderma species. 2022;15(10):2521-2532. https://doi.org/10.1111/1751-7915.14126

https://onlinelibrary.wiley.com/doi/10.1111/1751-7915.14126

REGULAR ISSUE

Open Access

De novo design of the global transcriptional factor Cra-regulated promoters enables highly sensitive glycolysis flux biosensor for dynamic metabolic control

  • Pages: 605-617
  • First Published: 20 December 2022
Description unavailable

Glycolysis is the primary metabolic pathway in all living organisms. Maintaining the balance of glycolysis flux and biosynthetic pathways is the crucial matter involved in the microbial cell factory. The glycolysis flux biosensor based on global transcription factors Cra that can sense in vivo fructose-1,6-diphosphate (FBP) was a powerful tool for fine-tuning and rewiring pathway flux. In order to avoid the influence on global metabolism generated by overexpression of global transcription factors, we designed and adjusted the high-performance positive- and negative-response biosensors using promoter do novo design for varied thresholds and dynamic ranges.

Open Access

Study of the persistence and dynamics of recombinant mCherry-producing Yarrowia lipolytica strains in the mouse intestine using fluorescence imaging

  • Pages: 618-631
  • First Published: 20 December 2022
Description unavailable

Yarrowia lipolytica is a dimorphic oleaginous non-conventional yeast widely used as a powerful host for expressing heterologous proteins. To study its potential for oral delivery of recombinant therapeutic proteins in mammals, we engineered two Y. lipolytica strains to produce the model fluorescent protein mCherry. We demonstrated that both Y. lipolytica strains transiently persisted in mice after four daily oral administrations and they maintained the active expression of mCherry in the mouse intestine. We used confocal microscopy and showed that Yarrowia cells were found essentially in the lumen and they were rarely in contact with epithelial cells while transiting through the ileum, caecum, and colon of mice. Taken as a whole, our results have shown that fluorescent Y. lipolytica strains constitute novel tools to study the persistence and dynamics of orally administered yeasts.

Open Access

Biocatalyst collection and heterologous expression of sesquiterpene synthases from basidiomycetous fungi: Discovery of a novel sesquiterpene hydrocarbon

  • Pages: 632-644
  • First Published: 28 December 2022

Description unavailable

Gene identification of basidiomycetous sesquiterpene synthases. Functional characterization of basidiomycetous sesquiterpene synthases. Discovery of the novel sesquiterpene hydrocarbon pleostene.

Open Access

Development of genetic tools for heterologous protein expression in a pentose-utilizing environmental isolate of Pseudomonas putida

  • Pages: 645-661
  • First Published: 24 January 2023
Description unavailable

Pseudomonas putida has emerged as a promising host for the conversion of biomass-derived sugars and aromatic intermediates into commercially relevant biofuels and bioproducts. Here we describe genetic tool development for P. putida M2, a soil isolate that can metabolize pentose sugars. These genetic tools provide a foundation to develop hosts complementary to P. putida KT2440 and expand the ability of this versatile microbial group to convert biomass to bioproducts.

Open Access

Left–Right-Position, party affiliation and regional differences explain low COVID-19 vaccination rates in Germany

  • Pages: 662-677
  • First Published: 09 January 2023
Description unavailable

This article investigates determinants of vaccine hesitancy based on political-ideological attitudes in Germany using data from four survey waves across >30,000 respondents (February until October 2021). Regression models show that party affiliation, political ideology and region of residence explain vaccine hesitancy. As a consequence, items capturing political-ideological attitudes should be integrated into contemporary vaccine assessment models.